PINK1 Polyclonal Antibody (PA5-86941) (2024)

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PINK1 Polyclonal Antibody (PA5-86941) (1)

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  • Primary Antibodies
  • PINK1 Antibodies

Invitrogen

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This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

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PINK1 Polyclonal Antibody (PA5-86941) (2)

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PINK1 Antibody (PA5-86941) in WB

Western blot was performed using Anti-PINK1 Polyclonal Antibody (Product # PA5-86941) and a 58 kDa band corresponding to PINK1 was observed across cell lines tested and increased upon Hydrogen Peroxide treatment. Whole cell extracts (20 µg lysate) of HeLa (Lane 1), A549 (Lane 2), SH-SY5Y (Lane 3) and SH-SY5Y treated with 25 mM of Hydrogen Peroxide for 24 hours (Lane 4) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001)... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more

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PINK1 Polyclonal Antibody (PA5-86941) (3)

PINK1 Polyclonal Antibody (PA5-86941) (4)

PINK1 Polyclonal Antibody (PA5-86941) (5)

PINK1 Polyclonal Antibody (PA5-86941) (6)

PINK1 Polyclonal Antibody (PA5-86941) (7)

PINK1 Polyclonal Antibody (PA5-86941) (8)

PINK1 Polyclonal Antibody (PA5-86941) (9)

Product Details

PA5-86941

Applications

Tested Dilution

Publications

Western Blot(WB)

1:500-1:2,000

-

Immunocytochemistry(ICC/IF)

1:200

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit/ IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide, corresponding to Human PINK1.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2803698

Target Information

PINK1 (PTEN-induced putative kinase 1) protects from mitochondrial dysfunction in cells by conferring different autophosphorylation activity to mitochondrial genes. The kinase domain of PINK1 appears to be critical in its protective effects. Mitochondrial proteins are critical for the regulation of cellular energy and adaptation to stress. Particularly in brain cells, mitochondrial demand is extraordinarily high, in order to accommodate aerobic and anaerobic support of high energy processes. When mitochondrial structure and/or function is disrupted in the brain, neurodegenerative diseases like Parkinson's disease are triggered.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases:BRPK; FLJ27236; PINK 1; PINK-1; protein kinase BRPK; PTEN-induced putative kinase protein 1; Serine/threonine-protein kinase PINK1, mitochondrial

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Gene Aliases:BRPK; PARK6; PINK1

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Molecular Function: PINK1 Polyclonal Antibody (PA5-86941) (10)non-receptor serine/threonine protein kinase PINK1 Polyclonal Antibody (PA5-86941) (11)protein modifying enzyme

Function(s)

mitophagypositive regulation of protein phosphorylationregulation of oxidative phosphorylationprotein phosphorylationubiquitin-dependent protein catabolic processresponse to stressresponse to oxidative stressmitochondrion organizationregulation of hydrogen peroxide metabolic processnegative regulation of gene expressionregulation of mitochondrion organizationpositive regulation of peptidase activitymacroautophagypositive regulation of macroautophagynegative regulation of macroautophagyphosphorylationprotein ubiquitinationpeptidyl-serine phosphorylationrespiratory electron transport chainregulation of protein ubiquitinationpositive regulation of protein ubiquitinationactivation of protein kinase B activitypositive regulation of synaptic transmission, dopaminergicpositive regulation of peptidyl-serine phosphorylationpositive regulation of dopamine secretioncellular response to oxidative stresspositive regulation of protein dephosphorylationintracellular signal transductionpeptidyl-serine autophosphorylationTORC2 signalingpositive regulation of I-kappaB kinase/NF-kappaB signalingregulation of protein complex assemblynegative regulation of neuron apoptotic processpositive regulation of translationnegative regulation of JNK cascadeprotein stabilizationpositive regulation of sequence-specific DNA binding transcription factor activitypositive regulation of ubiquitin-protein transferase activityregulation of mitochondrial membrane potentialpositive regulation of protein kinase B signalingregulation of proteasomal protein catabolic processcellular response to hypoxiaestablishment of protein localization to mitochondrionmaintenance of protein location in mitochondrionpositive regulation of mitochondrial fissionpositive regulation of release of cytochrome c from mitochondrianegative regulation of mitochondrial fissioncellular response to toxic substancemitophagy in response to mitochondrial depolarizationregulation of synaptic vesicle transportnegative regulation of autophagosome assemblypositive regulation of mitochondrial electron transport, NADH to ubiquinoneregulation of mitophagynegative regulation of mitophagynegative regulation of oxidative stress-induced cell deathnegative regulation of oxidative stress-induced neuron deathregulation of protein targeting to mitochondrionnegative regulation of hypoxia-induced intrinsic apoptotic signaling pathwaynegative regulation of hydrogen peroxide-induced neuron intrinsic apoptotic signaling pathwaynegative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxidepositive regulation of cristae formationpositive regulation of protein targeting to mitochondrionpositive regulation of free ubiquitin chain polymerizationregulation of reactive oxygen species metabolic processnegative regulation of reactive oxygen species metabolic processpositive regulation of ATP biosynthetic process

Process(es)

magnesium ion bindingprotease bindingprotein kinase activityprotein serine/threonine kinase activityprotein bindingATP bindingcalcium-dependent protein kinase activitykinase activitypeptidase activator activityubiquitin protein ligase bindingprotein kinase B bindingC3HC4-type RING finger domain binding

It has to be done as per old AB suggested Products section.

PINK1 Polyclonal Antibody (PA5-86941) (12)

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PINK1 Polyclonal Antibody (PA5-86941) (13)

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PINK1 Polyclonal Antibody (PA5-86941) (2024)
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