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- Primary Antibodies ›
- Apolipoprotein J Antibodies
Invitrogen
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
Cite Apolipoprotein J Polyclonal Antibody
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Apolipoprotein J Antibody (PA5-46931) in WB
Western blot was performed using Anti-Apolipoprotein J Polyclonal Antibody (Product # PA5-46931) and 38-75 kDa bands corresponding to Apolipoprotein J was observed across cell lines tested except Daudi, K-562 and 3T3-L1 fibroblasts. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Daudi (Lane 2), K-562 (Lane 3), 3T3-L1 (Lane 4) and 3T3-L1 differentiated to Adipocytes (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Produ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
Published figure supplied by
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_1.jpg?time=20220827 "Apolipoprotein J Polyclonal Antibody (PA5-46931) (6)")
_2.jpg?time=20220827 "Apolipoprotein J Polyclonal Antibody (PA5-46931) (7)")
Product Details
PA5-46931
Applications
Tested Dilution
Publications
Western Blot(WB)
0.1 µg/mL
View 1 publication1 publication
Immunohistochemistry (IHC)
-
View 1 publication1 publication
Immunohistochemistry (Frozen)(IHC (F))
5-15 µg/mL
-
Immunocytochemistry(ICC/IF)
5 µg/mL
View 1 publication1 publication
ELISA(ELISA)
0.1-0.4 µg/mL (Detection), 0.2-0.8 µg/mL (Capture)
-
Immunoprecipitation(IP)
25 µg/mL
View 1 publication1 publication
Product Specifications
Species Reactivity
Human,Mouse
Published species
Pig
Host/Isotype
Goat/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Clusterin Glu22-Glu448
Conjugate
Unconjugated
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2608081
Product Specific Information
In sandwich ELISAs, approximately 5% cross-reactivity with recombinant human Clusterin is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases:Aging associated protein 4; Aging-associated gene 4 protein; aging-associated protein 4; apo j; Apo J a; Apo J alpha; Apo J b; Apo J beta; Apo J α; Apo J β; Apo Ja; Apo Jb; Apo-J; ApoJ a; ApoJ alpha; ApoJ b; ApoJ beta; ApoJ α; ApoJ β; ApoJa; ApoJb; Apolipoprotein J; CLI; Clusterin; Clusterin alpha / beta chain; Clustrin; Complement associated protein SP40; Complement cytolysis inhibitor; complement lysis inhibitor; Complement-associated protein SP-40,40; GP80; HGNC:2095; Ku70-binding protein 1; Ku70-binding protein 1 (KUB1); MGC24903; NA1/NA2; SGP-2; Sulfated glycoprotein 2; Sulfated glycoprotein 2 (SGP2); testosterone repressed prostate message; Testosterone repressed prostate message 2 (TPRM2B); testosterone repressed prostate message-2; Testosterone-repressed prostate message 2; TRPM-2
Gene Aliases:AAG4; AI893575; APO-J; APOJ; CLI; CLU; CLU1; CLU2; D14Ucla3; KUB1; Msgp-2; NA1/NA2; SGP-2; SGP2; SP-40; Sugp-2; TRPM-2; TRPM2
UniProt ID:(Human) P10909, (Mouse) Q06890
Entrez Gene ID:(Human) 1191, (Mouse) 12759
Function(s)
cell morphogenesismicroglial cell activationcell deathpositive regulation of cell proliferationprotein importendocrine pancreas developmentcentral nervous system myelin maintenancepositive regulation of proteasomal ubiquitin-dependent protein catabolic processnegative regulation of protein hom*ooligomerizationpositive regulation of tumor necrosis factor productionpositive regulation of apoptotic processnegative regulation of apoptotic processpositive regulation of nitric oxide biosynthetic processpositive regulation of cell differentiationneuron projection morphogenesisprotein stabilizationpositive regulation of NF-kappaB transcription factor activitychaperone-mediated protein complex assemblyresponse to misfolded proteinchaperone-mediated protein foldingmicroglial cell proliferationintrinsic apoptotic signaling pathwayregulation of beta-amyloid clearanceregulation of neuron deathpositive regulation of neuron deathpositive regulation of beta-amyloid formationnegative regulation of intrinsic apoptotic signaling pathway in response to DNA damagenegative regulation of beta-amyloid formationregulation of neuronal signal transductionpositive regulation of tau-protein kinase activitypositive regulation of neurofibrillary tangle assemblypositive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic processpositive regulation of intrinsic apoptotic signaling pathwayrelease of cytochrome c from mitochondriaplatelet degranulationlipid metabolic processcomplement activationcomplement activation, classical pathwayagingresponse to light stimulusresponse to woundingresponse to virusresponse to potassium ionreverse cholesterol transportestrous cycleinnate immune responsecellular response to growth factor stimulus
Process(es)
protein bindingATPase activityubiquitin protein ligase bindingprotein N-terminus bindingchaperone bindingmisfolded protein binding
It has to be done as per old AB suggested Products section.
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