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- Primary Antibodies ›
- COL1A1 Antibodies
Invitrogen
View all (10) COL1A1 antibodies
Cite COL1A1 Polyclonal Antibody
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COL1A1 Antibody (PA5-86949) in ICC/IF
Immunofluorescence analysis of COL1A1 was performed using 70% confluent log phase IMR-90 and HT-29 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 5 minutes and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with COL1A1 Polyclonal Antibody (Product # PA5-86949) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Pro... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Product Details
PA5-86949
Applications
Tested Dilution
Publications
Western Blot(WB)
1:500-1:2,000
View 1 publication1 publication
Immunohistochemistry (Paraffin) (IHC (P))
1:50-1:200
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Product Specifications
Species Reactivity
Human,Mouse,Rat
Published species
Human
Host/Isotype
Rabbit/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide of human COL1A1.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2803705
Target Information
The protein encoded by this gene is the pro-alpha1 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases:alpha-1 type 1 collagen; Alpha-1 type I collagen; alpha1(I) procollagen; COL1A; Collagen 1; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; collagen of skin, tendon and bone, alpha-1 chain; collagen, type 1, alpha 1; collagen, type I, alpha 1; Collagen1A1; pro-alpha-1 collagen type 1; procollagen type I, alpha 1; procollagen, type 1, alpha 1; procollagen, type I, alpha 1; Type I Collagen; type I proalpha 1; type I procollagen alpha 1 chain; type I-alpha 1 collagen
Gene Aliases:Col1a-1; COL1A1; Cola-1; Cola1; COLIA1; EDSC; Mov-13; Mov13; OI1; OI2; OI3; OI4
UniProt ID:(Human) P02452, (Rat) P02454, (Mouse) P11087
Entrez Gene ID:(Human) 1277, (Rat) 29393, (Mouse) 12842
Molecular Function: 
extracellular matrix structural protein
Function(s)
skeletal system developmentblood vessel developmentosteoblast differentiationintramembranous ossificationendochondral ossificationblood coagulationvisual perceptionsensory perception of soundpositive regulation of epithelial to mesenchymal transitionnegative regulation of cell-substrate adhesionprotein transportplatelet activationextracellular matrix organizationcollagen fibril organizationpositive regulation of cell migrationcollagen catabolic processresponse to corticosteroidresponse to estradiolcollagen biosynthetic processprotein localization to nucleustooth mineralizationresponse to drugresponse to hydrogen peroxideresponse to peptide hormoneskin morphogenesiscellular response to fibroblast growth factor stimulustooth eruptionpositive regulation of transcription, DNA-templatedembryonic skeletal system developmentregulation of immune responseleukocyte migrationresponse to cAMPresponse to hyperoxiaface morphogenesisbone trabecula formationcartilage development involved in endochondral bone morphogenesisprotein heterotrimerizationcellular response to amino acid stimuluscellular response to mechanical stimuluscellular response to retinoic acidcellular response to vitamin Ecellular response to tumor necrosis factorcellular response to epidermal growth factor stimuluscellular response to transforming growth factor beta stimuluspositive regulation of canonical Wnt signaling pathwaycellular response to fluorideossificationresponse to nutrientresponse to mechanical stimulusresponse to nutrient levelswound healingskin developmentresponse to steroid hormoneskeletal system morphogenesisresponse to fluoride
Process(es)
extracellular matrix structural constituentprotein bindingidentical protein bindingmetal ion bindingplatelet-derived growth factor bindingmolecular_function
It has to be done as per old AB suggested Products section.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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